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Primary Research Applications Metabolic Pathway Research: Utilized in controlled in-vitro models to study metabolic regulation, energy balance, and intracellular signaling mechanisms

The existence of other IGF binding proteins, six in total, became clear when Hossenloop (20) developed Western ligand blotting as a technique to quantify these proteins

For example, testing one or both of the functional measures of vitamin B12, MMA (methylmalonic acid) and homocysteine, perhaps especially when values are in that so-called grey zone between 150 and 300 pmol/L or so

For references and more information , see the section on Vitamin B 12 in the Pregnancy and Lactation article